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Store part of the medium under continuous bright light, and the remaining medium under total darkness for 10 to 14 days, Inoculate both media, and observe growth, Trapping and consuming small nematodes Note the formation of a new conidium at the apex of the con i d i a l chain. Conidiophores sporangiophores erect or ascending, septate, giving rise above to coiled, fertile branches; sporocladia borne successively on the lower surface of the coils, septate, with narrowed apices, producing laterally pseudophialides that bear single sporangioles conidia ; conidia short-ellipsoid, not enveloped in liquid at maturity; saprophytic on dung. Setae straight or slightly bent, thick-walled, dark, independent or a sterile portion of conidiophore; phialides mostly terminal, straight, sometimes proliferating, forming conspicuous collarettes that are cupulate, or funnel-shaped, flaring; conidia phialospores hyaline, 1-celled sometimes to 4-celled , with a slender seta at each end; saprophytic on plant material or soil. This simple method often results in a high percentage of cultures free of contamination.
